INDUCTION AND MAINTENANCE OF EMBRYOGENIC CHARACTERISTICS OF CALLUS OF THE OIL PALM HYBRID MANICORÉ

ABSTRACT Large-scale oil palm propagation (Elaeis guineensis Jacq.) is difficult due to its unique apical meristem. In this context, micropropagation allows the multiplication of seedlings in vitro and storage germplasm elites. This study aimed induce embryogenic calluses from leaves plants low concentrations auxins observe maintenance these characteristics during cultivation. Calluses were induced 0.5 cm leaf explants Y3 culture medium supplemented with Picloram (4-Amino-3,5,6-trichloro-2-pyridinecarboxylic acid) or 2,4-D (2,4-dichlorophenoxyacetic acid), at 0, 1, 3, 6, 9 mg L-1. The callus appearance was subcultured evaluated regarding by cytochemical analyses. best treatment for induction composed 1mg.L-1 Picloram, which led 30% formation. classified into4 types, based on color morphology. cells nodular beige have characteristics, potential cell masses maintained over 20 months differentiated adaptation protocol can allow advance mass through tissue culture, indicating importance investigating topics proposed research.